tag:blogger.com,1999:blog-3017140681653842942.post1119975474040066508..comments2020-11-03T19:32:06.992-08:00Comments on J.A.M.M.Y.S: Lab TechniqueJ.A.M.M.Y.Shttp://www.blogger.com/profile/17180949601509896566noreply@blogger.comBlogger4125tag:blogger.com,1999:blog-3017140681653842942.post-83124273506836471792007-09-16T07:31:00.000-07:002007-09-16T07:31:00.000-07:00sorry michelle, ignore my 1st quest.. i nv saw the...sorry michelle, ignore my 1st quest.. i nv saw the reply u made after i post my comment..<BR/><BR/>Ai Teewe are the XiaoBianTai-7!https://www.blogger.com/profile/08103713119175158043noreply@blogger.comtag:blogger.com,1999:blog-3017140681653842942.post-29209367279776012612007-09-16T07:29:00.000-07:002007-09-16T07:29:00.000-07:00hey michelle,what is the purpose of formamide at t...hey michelle,<BR/><BR/>what is the purpose of formamide at the last part of your DNA sequencing?<BR/>by the way, u all purify the PCR products by enzymes? i use the purification kit in my lab from the manufacturer, is there a difference in the principle?<BR/><BR/>AI Tee<BR/>0503160Dwe are the XiaoBianTai-7!https://www.blogger.com/profile/08103713119175158043noreply@blogger.comtag:blogger.com,1999:blog-3017140681653842942.post-7533233784825263592007-09-14T00:20:00.000-07:002007-09-14T00:20:00.000-07:00hiya kent,the function of the sodium acetate and E...hiya kent,<BR/>the function of the sodium acetate and EDTA us to terminate the cycle sequencing process.<BR/>We used Hi-Di formamide which is a highly deionized formamide to dissole the DNA pellets before placing the sequecning plates into the sequencer machine.<BR/><BR/>michelle tg01 0503808HJ.A.M.M.Y.Shttps://www.blogger.com/profile/17180949601509896566noreply@blogger.comtag:blogger.com,1999:blog-3017140681653842942.post-78835388846752270642007-09-10T07:56:00.000-07:002007-09-10T07:56:00.000-07:00Hi,Would like to ask what is the fuction of the so...Hi,<BR/><BR/>Would like to ask what is the fuction of the sodium acetate and EDTA in the PCR master mix, and what formaldehyde is used for in the sequencing reaction. <BR/><BR/>Thanks!<BR/>Kent<BR/>TG01Kenthttps://www.blogger.com/profile/01254696635743571323noreply@blogger.com