tag:blogger.com,1999:blog-3017140681653842942.post4905398623648785408..comments2020-11-03T19:32:06.992-08:00Comments on J.A.M.M.Y.S: Lab TechniquesJ.A.M.M.Y.Shttp://www.blogger.com/profile/17180949601509896566noreply@blogger.comBlogger3125tag:blogger.com,1999:blog-3017140681653842942.post-9182526159934178262007-08-09T00:38:00.000-07:002007-08-09T00:38:00.000-07:00For Ci Liang:Some of the disadvantages of IEF:1. T...For Ci Liang:<BR/><BR/>Some of the disadvantages of IEF:<BR/>1. The IEF gels are expensive,<BR/>2. The electrophoresis takes about 16 hours, time consuming, <BR/>3. Some highly hydrophobic proteins are lost during IEF as they are not solubilize.<BR/>4. A small amount of protein might be precipitated due the overlapping (see diagram in blog entry).<BR/><BR/>If we only use SDS PAGE method the proteins are only separated by molecular weight. This is a 1 Dimension separation What we like to have a more refined separation thus we use the 2Dimension separation.<BR/><BR/><BR/>For KangTing:<BR/><BR/>IPG stands for Immobilized pH Gradient. The strip is sold in dehydrated form thus it has to be rehydrated to open up the pores of the gel allowing the proteins to enter. <BR/><BR/>Hope it answers your questions and cleared your doubt. =D<BR/><BR/>take care!J.A.M.M.Y.Shttps://www.blogger.com/profile/17180949601509896566noreply@blogger.comtag:blogger.com,1999:blog-3017140681653842942.post-20003224287969824742007-08-05T18:24:00.000-07:002007-08-05T18:24:00.000-07:00Hello =)Er, what does IPG stands for?And why does ...Hello =)<BR/><BR/>Er, what does IPG stands for?<BR/>And why does the strip has to be rehydrated?<BR/><BR/>Thanks!<BR/><BR/>Chen Kangting<BR/>0503331A<BR/>TG02royal physicianshttps://www.blogger.com/profile/17022912731511892610noreply@blogger.comtag:blogger.com,1999:blog-3017140681653842942.post-91630841073933960632007-08-05T08:18:00.000-07:002007-08-05T08:18:00.000-07:00hello!Is there any disadvantages of using Isoelect...hello!<BR/><BR/>Is there any disadvantages of using Isoelectric Focusing (IEF)? If don't use IEF but only SDS PAGE, will the results be affected? Thanks<BR/><BR/>Ci Liang<BR/>TG01BloodBank.MedMic.Haematologyhttps://www.blogger.com/profile/11938959656718537358noreply@blogger.com