tag:blogger.com,1999:blog-3017140681653842942.post5551121757366938359..comments2020-11-03T19:32:06.992-08:00Comments on J.A.M.M.Y.S: Lab TechniquesJ.A.M.M.Y.Shttp://www.blogger.com/profile/17180949601509896566noreply@blogger.comBlogger3125tag:blogger.com,1999:blog-3017140681653842942.post-75350394447811303682007-08-18T07:51:00.000-07:002007-08-18T07:51:00.000-07:00Royston,The proteins are compared using PDQuest, t...Royston,<BR/><BR/>The proteins are compared using PDQuest, the software that i had mentioned. Usually, we will run triplicate gels. As we have to compare the proteins between 2 bacteria strains, we will have a total of 6 gels. Using the software, a master gel will be created. A master gel is a virtual gel created by selecting a gel from the 6 gels that has the best quality. This selection can be done by the software or by the user. Protein spots that are found on other gels but not the gel that is selected to be the master gel can be added to the master gel. The 6 gels will then be compared against the master gel. Then you will be able to analyse the proteins qualitatively, where you can see the spots that are present in some gels but not the other gels. For quantitatively, you can select spots that differ in the intensity and use a function in the software to quantify the difference in concentration. Yes we use protein markers but not for recognition. As protein markers have a known molecular weight and concentration, we can use that as a basis to determine the Mw and concentration of the proteins. For the recognition of proteins, it is done by the MALDI, where the proteins are sequenced and identified. Sorry for the lengthy answer. Hope you understand=]<BR/><BR/>Ming BoonJ.A.M.M.Y.Shttps://www.blogger.com/profile/17180949601509896566noreply@blogger.comtag:blogger.com,1999:blog-3017140681653842942.post-78571448563235300402007-08-18T07:18:00.000-07:002007-08-18T07:18:00.000-07:00Hi mingboon,Ask u ah, how do you all recognise and...Hi mingboon,<BR/><BR/>Ask u ah, how do you all recognise and compare the protein spots on different gels? Do you use any protein markers for recognition?<BR/><BR/>Royston Tan<BR/>TG01The Lab Freakshttps://www.blogger.com/profile/04322986402512975861noreply@blogger.comtag:blogger.com,1999:blog-3017140681653842942.post-45872672434022941372007-08-18T07:13:00.000-07:002007-08-18T07:13:00.000-07:00This comment has been removed by the author.The Lab Freakshttps://www.blogger.com/profile/04322986402512975861noreply@blogger.com